Table 1 Steady-state kinetic parameters of mosquito proteases and bovine trypsin using BApNA as a substrate

Protease ^a

K _M(BApNA) (μM)

k _cat (s ^-1 )

k _cat /K _M (mM ^-1 s ^-1 )

(μmol min ^-1 (mg of protein) ^-1 )

AaET

63.0 ± 1.3

2.03

32.3

5.08

AaSPVI

11.8 ± 0.3

0.16

13.5

0.40

AaSPVII

19.2 ± 1.0

0.35

18.5

0.88

AaLT

ND

ND

ND

ND

Bovine Trypsin

1051 ± 54

1.30

1.2

3.28