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Figure 1 | BMC Biochemistry

Figure 1

From: The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor α with the small GTPase Rac

Figure 1

Fer forms a complex with RhoGDIα and phosphorylates it on tyrosine. (A) COS-1 cells were transfected with Bcr/Abl or Fer (pSG5-Fer). Autoradiogram of immune complex kinase reaction performed with immunoprecipitated Bcr/Abl or Fer (CH-6 antibodies) as indicated, with bacterially expressed GST-RhoGDIα or control GST added. (B) Immunoprecipitation (IP) from cells transfected with pEGFP tagged-Fer-WT or Fer-MT alone or together with RhoGDIα using RhoGDIα antibodies (Santa Cruz) (left panel) or Fer monoclonal rat antiserum (right panel). Antibodies used for immunoblot (IB) are indicated below the panels. After reaction with the pY20 antibodies, blots were stripped and reacted with RhoGDIα (Santa Cruz) or GFP antibodies. Arrowheads point to the locations of Fer and RhoGDIα. Molecular weight standards are in kDa. (C) Co-immunoprecipitation of endogenous Fer (using antibodies from Abcam) and RhoGDIα (Santa Cruz antibodies) proteins in RBL-2H3 mast cell lysates. These results are representative of three independent experiments.

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BMC Biochemistry

ISSN: 1471-2091

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