Figure 2

Comparison of pepG1 with well-known family G1 peptidase and putative bacterial and archaeal members. Full-length sequences including signal peptides were aligned using ClustalX version 2.0.11. The residues numbering for each peptidase is indicated. The seven highly conserved segments in all G1 peptidases are colored according to the percentage of the residues in each column that agrees with the consensus sequence. Only the residues that agree with the consensus residue for each column are colored. Dark blue means > 80%, blue > 60%, light blue > 40% and white < 40%. The catalytic dyad is colored red and the residues involved in a highly conserved disulfide bridge are shown in yellow. The fungal peptidases used for the alignment were aspergilloglutamic peptidase (AGP, [GenBank: P24665]) from Aspergillus niger, scytalidoglutamic peptidase (SGP, [GenBank: P15369]) from Scytalidium lignicolum, acid peptidases B and C (EapB [GenBank: Q00550] and EapC [GenBank: Q00551]) from Cryphonectria parisitica, Penicillium marneffei acid proteinase (PMAP-1, [GenBank: EEA28697]), BcACP1 ([GenBank: AAZ77775) from Botryotinia fuckeliana and Talaromyces emersonii glutamic peptidase 1 (TGP1, [GenBank: Q8X1C6]). The putative bacterial peptidases were [GenBank: YP_003762485] from Amycolatopsis mediterranei (Ame), [GenBank: ACB95479] from Beijerinckia indica (Bin), [GenBank: ABO59772] from Burkholderia vietnamiensis (Bvi), [GenBank: YP_003114490] from Catenulispora acidiphila (Cat), [GenBank: BAH07727] from Clostridium kluyveri (Ckl) and [GenBank: ABY24309], [GenBank: YP_003244752] from Geobacillus sp. (Geo), [GenBank: HM011103] from Alicyclobacillus sp. DSM 15716 (pepG1) and [GenBank: ABY21885] from Renibacterium salmoninarum (Rsa_1 and Rsa_2). The two archaeal peptidases were [GenBank: YP_003816089] from Acidilobus saccharovorans (Asa) and [GenBank: ABW02092] from Caldivirga maquilingensis (Cma).